Genome-wide maps of chromatin state in pre-osteoblastic and lineage-committed cells.

Epigenetic mechanisms regulate osteogenic lineage differentiation of mesenchymal stromal cells. Histone methylation is an important step in controlling local chromatin structure and gene expression and it is controlled by multiple lysine demethylases. Although Lsd1 knockdown did not affect global H3K4 methylation levels, genome-wide ChIP-Seq analysis revealed high levels of Lsd1 at gene promoters and its binding was associated with di- and tri-methylation of histone 3 at lysine 4 (H3K4me2 and H3K4me3) Examination of 4 different histone modifications and Lsd1/Kdm1a occupancy in MC3T3-E1 at different stages of differentiation