Mouse hearts after myocardial infarct

Data set contains samples from isolated cells of murine hearts after myocardial infarction (LAD surgery). We used an endothelial tracing system to mark endothelial cells with EGFP prior to infarct. In brief, Cdh5-ERT2+/-;mT/mG mice, which were used at an age of 10-12 weeks. Cre expression was induced by daily intraperitoneal injections of 2 mg tamoxifen for 1 week in all mice. One week after tamoxifen injection, left anterior descending coronary artery ligation surgery was performed, inducing myocardial infarction. We collected samples at homeostasis, days(d) 1, 3, 7, 14 and 28 after infarction and isolated the non-cardiomyocyte fraction for single cell sequencing. Murine scRNA-seq libraries were prepared using Chromium Single Cell 3′ v3 Reagent Kit (10X Genomics), according to manufacturer’s protocols. Raw reads were mapped against a customized version of mm10 (GRCm38.p4) containing sequences of EGFP and tdTomato.

本数据集包含经左前降支（Left Anterior Descending, LAD）手术构建心肌梗死模型后，小鼠心脏的分离细胞样本。我们在梗死造模前，采用内皮细胞示踪系统以增强绿色荧光蛋白（EGFP）标记内皮细胞。简言之，实验使用10~12周龄的Cdh5-ERT2+/-;mT/mG小鼠。对所有小鼠每日腹腔注射2mg他莫昔芬（tamoxifen），持续1周以诱导Cre重组酶表达。他莫昔芬注射一周后，实施左前降支冠状动脉结扎术以构建心肌梗死模型。我们分别在稳态条件下、梗死后第1、3、7、14及28天采集样本，分离非心肌细胞组分用于单细胞测序。小鼠单细胞RNA测序（scRNA-seq）文库按照制造商提供的实验方案，使用Chromium单细胞3′端v3试剂试剂盒（10X Genomics）制备。原始测序读数将与包含EGFP和tdTomato序列的定制化mm10（GRCm38.p4）基因组版本进行比对。

搜集汇总

背景与挑战

背景概述

该数据集包含心肌梗死手术后小鼠心脏非心肌细胞部分的单细胞测序数据，使用Cdh5-ERT2+/-;mT/mG小鼠模型，并在不同时间点收集样本。数据集采用CC BY 4.0许可证，适用于心血管疾病研究。