Absolute and relative quantification of 16S rRNA, nirK and nirS genes in both estuarine sediment types (HBM and LBM).
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Analyses was performed in triplicate for each biological replicate (n = 3). Gene copy numbers given were corrected for PCR inhibition.a. Detection limit, 101 copies; PCR efficiency, 90.4%; standard curve R2, 0.99; inhibition factor range: 0.07–0.10.b. Detection limit, 101 copies; PCR efficiency, 87%; standard curve R2, 0.99; inhibition factor range: 0.36–0.62.c. Detection limit, 101 copies; PCR efficiency, 62.9%; standard curve R2, 0.99; inhibition factor range: 0.31–0.55.Absolute and relative quantification of 16S rRNA, nirK and nirS genes in both estuarine sediment types (HBM and LBM).
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2015-12-03
