CIL:12416

The images in this group show electron micrographs of epidermal and mesophyll cells in thin sections of maize leaves infected with Maize mosaic virus (MMV, Rhabdoviridae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. This tif image is of a mesophyll leaf cell from a maize plant infected with Maize mosaic virus (MMV), and is identical to the labeled jpg file CIL:16309. MMV virions, budding through the inner nuclear membrane, are accumulating between this membrane and the outer nuclear membrane as well as in intracytoplasmic vesicles, sometimes forming crystalline arrays. Detailed methods: Pieces of leaves were processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. See E.-D. Ammar et al. (2005) J. Phytopathology, 153:129-136.