Thermodynamic parameters of PGT 121 antibodies and CD4 binding to gp120 and SOSIP.664 trimers measured by isothermal titration calorimetry.

%Reported values are averages from at least two independent measurements and associated errors are approximately 10% of the average. Representative isotherms can be found in Figs. S6, S7.*The binding isotherms do not allow to accurately determining these binding parameters.#The change in Gibbs free energy (ΔG) was determined using the relationship: ΔGbinding = RTlnKd[75].&The stoichiometry of binding (N) is directly affected by errors in protein concentration measurements, sample impurity and glycan heterogeneity on gp120.