siRNA-knockdown.

Values: mRNA expression mean [%] ± SEM of NonTarget control (n≥4).Knockdown by specific siRNA pools transfected with D4 (Dharmacon transfection reagent) in HepG2 cells. Single knockdowns were performed for FOXO1, FOXO3a, SIRT1, AKT, or NRF2 (n = 8) and double knockdowns for combinations FOXO1/FOXO3a, FOXO1/SIRT1, FOXO1/AKT, FOXO1/NRF2, FOXO3a/Sirt1 or NRF2/AKT (n = 4) using each half of the amount of siRNA used in transfection-mixes. 48 h after transfection of cells including a starvation period of 16 h in EMEM without FBS, HepG2 cells were treated with DMSO 0.5% for 24 h (mock stimulation), RNA extracted, reverse transcribed and qRT-PCR performed with primers given in table 1. Quantified relative amplification vs housekeeping gene RPL32 expression was normalized to the rate in HepG2 transfected with non-target NT siRNA (100% rate of expression).