Transcriptome sequencing (RNAseq) of pooled aphid genotypes in response to crowded conditions

The goal of this study was to compare how pea aphids (Acyrthosiphon pisum) with different phenotypes (those that produced a high proportion of winged offspring in response to crowded conditions vs. those that produced a low proportion of winged offsrping) differed in their transcriptional response to crowding. 10 genotypes of each phenotype were pooled from two treatments (solitary vs. crowded conditions), across 4 biological replicates. Offspring were dissected out of adults, and adult carcasses were used for RNA extraction.