Effect of inhibitors and inducers of cytochrome P450 isoenzymes on the cytotoxicity of A398.

Cell viability was assessed by the MTT method after 24 h of exposure. The treatment of HepG2 cells with A398 was done in the absence or presence of dexamethasone (inductor CYP3A), ketoconazole (inhibitor CYP3A) or borneol (inhibitor UGTs). In the latter condition, cells were pretreated with 10 µM of dexamethasone (for 24 h) or co-incubated with 15 µM ketoconazole or with 500 µM borneol. Each IC50 value represented mean ± SE of three independent experiments.Effect of inhibitors and inducers of cytochrome P450 isoenzymes on the cytotoxicity of A398.