Microbial Observatory at North Temperate Lakes LTER High-resolution temporal and spatial dynamics of microbial community structure in freshwater bog lakes 2005 - 2009 original format (Reformatted to the ecocomDP Design Pattern)

This data package is formatted as an ecocomDP (Ecological Community Data Pattern). For
more information on ecocomDP see https://github.com/EDIorg/ecocomDP. This Level 1 data
package was derived from the Level 0 data package found here:
https://pasta.lternet.edu/package/metadata/eml/knb-lter-ntl/349/4. The abstract below was
extracted from the Level 0 data package and is included for context: The North Temperate Lakes - Microbial Observatory seeks to study freshwater microbes
over long time scales (10+ years). Observing microbial communities over multiple years using
DNA sequencing allows in-depth assessment of diversity, variability, gene content, and
seasonal/annual drivers of community composition. Combining information obtained from DNA
sequencing with additional experiments, such as investigating the biochemical properties of
specific compounds, gene expression, or nutrient concentrations, provides insight into the
functions of microbial taxa. Our 16S rRNA gene amplicon datasets were collected from bog
lakes in Vilas County, WI, and from Lake Mendota in Madison, WI. Ribosomal RNA gene amplicon
sequencing of freshwater environmental DNA was performed on samples from Crystal Bog, North
Sparkling Bog, West Sparkling Bog, Trout Bog, South Sparkling Bog, Hell’s Kitchen, and Mary
Lake. These microbial time series are valuable both for microbial ecologists seeking to
understand the properties of microbial communities and for ecologists seeking to better
understand how microbes contribute to ecosystem functioning in freshwater.