Altered thymic NKT1 and NKT2 differentiation in murine haploidentical bone marrow transplantation with chronic GvHD. Altered thymic NKT1 and NKT2 differentiation in murine haploidentical bone marrow transplantation with chronic GvHD

To investigate whether intermediate iNKT precursor cells were affected in GvHD mice along the differentiation journey toward mature effector cells, we performed scRNA-seq on thymic iNKT precursors from BMT mice with and without cGvHD and non-BMT mice. We identified Multiple early and late NKT1 precursor clusters and further explored the development of NKT1 under physiological as well as transplantation condition. Overall design: We performed scRNA-seq on thymic TCRβ+CD1d-tet+CD24-NK1.1- iNKT precursors from BMT mice with and without cGvHD and non-BMT mice. We identified Multiple early and late NKT1 precursor clusters and further explored the development of NKT1 under physiological as well as transplantation condition.