Yeast strains used in this work.
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a)strain GAL-RPS15 [6] transformed with a PCR holding the rps15-1 allele (kindly from P. Milkereit, unpublished). A thermo-sensitive clone was isolated and cured from pFL38-GAL::RPS15 plasmid. rps15-1 locus was sequenced for confirmation. b)transformed with PCR product amplified from the Δsnr36::URA3 strain from [29], kindly provided by E. Fayet-Lebaron. Absence of SnR36 was checked by Northern blotting.
创建时间：
2013-02-21
