Metal availability predicts community metallogenomes in marine oxygen minimum zones. marine metagenome

Marine oxygen minimum zones (OMZs) are hotbeds of microbial diversity and biogeochemical cycling. The factors structuring OMZ microbial gene content and metabolic potential are complex and not well understood. This project sampled the major anoxic OMZ of the Eastern Tropical North Pacific to explore the hypothesis that trace metal availability shapes microbial gene content. Seawater for microbial metagenome was collected in June 2013 from multiple depths along the vertical OMZ redox gradient at two sites off Manzanillo, Mexico: Station 6 (nearshore; 18.9°N, 104.5°W; 30, 85, 100, 125, 300 m depths) and Station 10 (offshore; 18.8°N, 104.7°W; 30, 80, 125, 300 m depths). Community microbial DNA was extracted and shotgun sequenced using Illumina MiSeq technology (250X250 paired end) and analyzed relative to corresponding trace metal concentration measurements. De-multiplexed, un-merged sequence reads representing nine metagenomic datasets are deposited in the SRA in association with this accession number.