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Amplification reactions using lambda phage DNA

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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA756278
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资源简介:
Advances in long read sequencing motivates the development of new methods for DNA amplification. The T7 replisome is a multiprotein complex capable of highly processive DNA synthesis, and was used in DNA amplification reactions of lambda phage genomic DNA. Nanopore sequencing uncovered a relationship between polymerase exonuclease activity and template-switching in the amplification reaction, and provided a mechanistic description of the process.
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2021-08-19
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