RdRP-induced antiviral phenotype is mediated in a MDA5-MAVS, IFNαβR-dependent manner.

(A-E) Knockout mice were bred to RdRP mice and the antiviral phenotype of each cross was analyzed by immunoblotting and survival. Left, cerebrum (top panel), spinal cord (middle), and cerebellum (bottom) tissue homogenates (n = 2 uninfected mice per genotype, 1 per lane) were analyzed by immunoblotting. Right, survival curve following EMCV infection (n = 8 mice per genotype), data are representative of two independent experiments. (A) IfnγR-/- cross. (B) IfnαβR-/- cross. (C) Tlr3-/- cross. (D) Mda5-/- cross. (E) Mavs-/- cross.