Anti-lysozyme Silverbody Data 2022

Molecular conformational engineering is to engineer flexible non-functional molecules into unique conformations to create novel functions just like natural proteins fold. Obviously, it is a grand challenge with tremendous opportunities. Based on the fact that natural proteins are only marginally stable with a net stabilizing energy of about two hydrogen bonds, we proposed that the energy barrier for the adatom diffusion can serve as a general replacement of protein scaffolds for the conformational engineering of protein fragments, i.e. to restore the native conformations of protein fragments on nanoparticle surfaces. To prove this hypothesis, herein, we successfully restore the antigen-recognizing function of the flexible peptide fragment of a natural anti-lysozyme antibody on the surface of silver nanoparticles, creating a silver nanoparticle-base artificial antibody (Silverbody). A plausible mechanism is proposed, and some general principles for conformational engineering are summarized to guide the future studies in this new area.

分子构象工程旨在将柔性非功能分子塑造成独特的构象，以创造出类似天然蛋白质折叠的新功能。显而易见，这是一项充满重大挑战与无限机遇的宏伟任务。鉴于天然蛋白质仅凭借约两个氢键的净稳定能维持微弱稳定性，本研究提出将吸附原子扩散的能量势垒作为蛋白质支架的一般替代品，用于蛋白质片段的构象工程，即恢复蛋白质片段在纳米粒子表面的天然构象。为验证此假设，本研究成功恢复了天然抗溶菌酶抗体柔性肽片段在银纳米粒子表面的抗原识别功能，从而创建了基于银纳米粒子的仿生人工抗体（Silverbody）。提出了一种合理的机制，并总结了构象工程的一些通用原则，以指导该新领域未来的研究工作。