Gene expression analysis following overexpression of L2 delta13 in C57BL/6J mice

High level expression of LOXL2 has been shown to be associated with tumor metastasis and poor clinical outcome in several types of cancers. We have previously identified a natural LOXL2 splice variant, called L2 delta13, in human esophageal squamous cell carcinoma cells. Unlike extracellular full-length LOXL2, L2 delta13 lacks amine oxidase activity and fails to be secreted from cells. To determine which genes are regulated by L2 delta13 in vivo, next generation RNA sequencing was performed to compare the RNA expression profile of liver tissues excised from L2 delta13-overexpressing and wild-type mice. RNA-sequencing of overexpression of L2 delta13 and wild type mice were performed by the BGISEQ-500 platform.