Differential expression of mRNAs in IFN-gamma treated HTR-8/SVneo versus untreated control by next generation sequencing

The goal of this study is to compare genes expressed by IFN-gamma treated HTR-8/SVneo cells to genes expressed in untreated control HTR-8/SVneo cells to identify genes which play a role during IFN-gamma-mediated HTR-8/SVneo cells invasion Overall design: cDNA libraries were made from total RNA of untreated control and 24 h IFN-gamma treated samples by TruSeq RNA Library Prep Kit v2. Deep sequencing of cDNA libraries were performed with the help of Illumina Genome Analyzer IIx. Raw sequence data was imported into the CLC Genomics Workbench 6.5.1. software. The sequence reads were trimmed for adapter sequences and low quality base. The trimmed raw sequences were subjected to mRNA-sequence analysis, by mapping them to Human Genome GRCH37.p.13 .