RNA sequencing of whole mouse liver from BDL, CCl4 and Western diet + CCl4-injury models of liver fibrosis

Liver fibrosis is the pathological consequence of liver injury, resulting in excessive accumulation of ECM proteins, such as collagen. To investigate how lipid metabolic pathways are dysregulated in liver fibrosis, we selected three mouse models. The BDL model is widely used to induce biliary fibrosis. Chronic CCl4 treatment is a highly reproducible and recognised model of liver disease, with similar histological and biochemical changes to human disease. In addition, a WD+CCl4 model was included to recapitulate some of the features of metabolic dysfunction-associated steatotic liver disease (MASLD). The use of these models enabled us to link gene expression analysis and dysregulated ipid metabolism in fibrogenesis. Publication Gruevska A. et al. Spatial lipidomics reveals sphingolipid metabolism as anti-fibrotic target in the liver. https://doi.org/10.1016/j.metabol.2025.156237.