RNA-Seq of safflower under drought

RNA were extracted from leaf samples of no-treatment (control) and drought treatment, mRNA was capturedand cDNA was synthesized. Then using Illumina Novaseq 6000 the samples were sequenced by paired method and short reads were obtained. The quality of reads was done and them trimmed to obtain clean reads, and again the quality of clean reads was checked. Clean short reads were mapped on indexed genome reference of safflower and transcript counts were obtained. After normalization for library size in all samples, the differentially expressed genes (DEGs) in drought-treated samples relative to normal samples were identified.