AMR Benchmarking dataset - Metagenomics

Metagenomic benchmarking dataset for AMR detection pipelines for assemblies focusing on ESKAPE pathogens in addition to Salmonella. This dataset consists of closed genomes from NCBI where paired-end Illumina data was available. These genomes were then randomly assigned a relative abundance, had additional AMR genes randomly inserted (to cover all AMR genes in CARD v3.1.4) and metagenomic Illumina reads simulated from them.<br> <br> Metagenomic simulation was performed using: https://github.com/fmaguire/AMR_Metagenome_Simulator and the entire process can be repeated using the metagenome_benchmark.sh script included above.<br>