RNA-Seq of UXT KO in Primary Sertoli cells

We created a knockout model of ART-27(UXT) specially in the Sertoli cells of the mouse testes. In order to see how loss of ART-27 effects the Sertoli cell transcriptome we isolated Sertoli cells from WT and KO animals. The WT cells were infected with a vector only construct, and the KO cells were infected with CRE producing construct. examination of transcriptional changes in WT primary Sertoli cells and KO Primary Sertoli cells. Each condition was done in triplicate