Phosphodiesterase 10A (PDE10A) as a novel target to suppress β-catenin and RAS signaling in ovarian cancer

The goal of this study was to investigate molecular pathways associated with PDE10A in ovarian cancer. We used CRISPR/Cas9 to knockout PDE10A in SKOV3 cells. We used the Illumina NextSeq500 to generate between 30-35 million paired end 75bp sequencing reads per sample for transcript level abundance. Comparison of SKOV3 parental and Empty Vector clone 1B9 (EV 1B9) vs. SKOV3 PDE10A KO clones 5H5 and 2F4 (two biological replicates per cell line)