RNA sequencing of infected and uninfected WT and HIF-1ɑ-/- peritoneal macrophages

The goal of this study is to perform transcriptome profiling of both infected and uninfected WT and HIF-1ɑ-/- peritoneal macrophages using RNA sequencing techniques. For that, WT and HIF-1ɑ-/- peritoneal macrophages were infeced for 6 hours with L. donovani promastigotes (or left uninfected as a control) and RNA samples were stored in TRI reagent (Sigma), for further analysis. Samples from 5 WT and 5 HIF-1ɑ-/- mice were analyzed