The non-apoptotic function of Caspase-8 in negatively regulating the CDK9 mediated Ser2 phosphorylation of RNA Polymerase II in cervical cancer

Analysis of the gene expression profile of the cervical cancer cell line HeLa in the presence or absence of Caspase-8 expression. The result offered an useful insight into the altered gene expression of metastasis associated genes due to the negative regulation of RNAPII mediated gene transcription by Caspase-8 Total RNA from non-synchronized and S/G2-phase synchronized HeLa WT and KO cells were extracted using the RNeasy Plus kit (Qiagen) and subjected to microarray expression profiling using the HumanHT-12 v3 arrays. Additional controls were also included in the form Caspase-8 knock-down HeLa WT cells and Caspase-8 overexpressing KO cells