Bile's chronic effect on the Cystic Fibrosis lung pathogen Pseudomonas aeruginosa

Many patients with Cystic Fibrosis suffer from Gastroeosphgeal Refux Disease (GERD). This disease may lead to the aspiration of bile into the lungs. We invesigated the effect of bile on the CF lung pathogen P. aeruginosa. We analysed gene expression profiles, comparing bile treated and untreated cells to identified distinct classes of up/down-regulated genes. P. aeruginosa PAO1 was grown to log stage for RNA extraction and hybridization on Affymetrix microarrays. We conducted three biological replicates as to increase the resolution of expression profiles.