Adipose derived stem cells (ASCs) methylation differences between Crohn Disease and No-Crohn individuals

Pathological expansion of adipose tissue (AT) is supported by adipocyte precursors, termed adipose-derived stem cells (ASCs). Elucidation of mechanisms underlying ASC function may lead to therapeutic interventions to use in cell therapy treatments on Crohn’s disease patients. Using epigenome-wide association studies, we explored the impact of Crohn’s disease on the methylation signature of human ASCs Genomic DNA was extracted from cells using the NucleoSpin® Tissue Kit (Macherery-Nagal GmbH). DNA methylation profiles were generated on the Infinitum Human-EPIC Methylation850K BeadChip (Illumina). The BeadChip was developed to assay more than 850,000 CpG sites and selected CpG loci in parallel. DNA methylation data were processed using GenomeStudio software (Illumina) by applying the default settings.