Autoimmunity associated allele of tyrosine phosphatase gene PTPN22 enhances anti-viral immunity

In this study, we examine gene expression and function of immune cell subsets to demonstrate how a common allelic variant of PTPN22, which strongly increases the risk of autoimmune disease, promotes successful clearance of an otherwise chronic viral infection. At 8 days post infection, splenocytes from 4-5 age matched, sex matched PEP-WT Wild Type and PEP-619WW Mutant mice were removed, processed , and resuspended in a single cell suspension. Splenocytes from each genotype were pooled and FACS sorted for Live CD45+ cells. Sorted cells were analyzed using single cell RNA sequencing via 10x Genomics platform