Failure to mate enhances investment in behaviors that may promote mating reward and impairs the ability to cope with stressors via a subpopulation of Neuropeptide F receptor neurons

Living in dynamic environments such as the social domain, where interaction with others determines the reproductive success of individuals, requires the ability to recognize opportunities to obtain natural rewards and cope with challenges that are associated with achieving them. As such, actions that promote survival and reproduction are reinforced by the brain reward system, whereas coping with the challenges associated with obtaining these rewards is mediated by stress-response pathways, the activation of which can impair health and shorten lifespan. While much research has been devoted to understanding mechanisms underlying the way by which natural rewards are processed by the reward system, less attention has been given to the consequences of failure to obtain a desirable reward. As a model system to study the impact of failure to obtain a natural reward, we used the well-established courtship suppression paradigm in Drosophila melanogaster as means to induce repeated failures to ob..., This dataset includes all the experiments done in this particular manuscript and include: FlyBowl raw data, QPCR, aggression, Mating duration, sensitivity to stress curves, activity monitor and metabolomics, , # Failure to mate enhances investment in behaviors that may promote mating reward and impairs the ability to cope with stressors via a subpopulation of Neuropeptide F receptor neurons.

Give a brief summary of dataset contents, contextualized in experimental procedures and results.

## Description of the data and file structure

This dataset contains all the raw data for this particular manuscript. It includes per-fly and per-frame data for the experiments done using the Flybowl system, QPCR data, metabolomic data, aggression, sleep, mating and courtship suppression data. The file name describes the relevant figure and genotype.

Empty cells contain n/a.

**Below is the list of the files:**

1. Courtship suppression data-set relevant for Figure S3. The table contains manual scoring of various courtship related actions (number of events and duration). This includes latency to first courtship action (sec), overall time spend courting (sec), latency to first attempt of copulation (sec)...