miRNA profiling in RAW264.7 cells treated without or with LPS and IFNγ

We have identified a number of miRNAs that are differentially expressed in LPS and IFNγ treated RAW264.7 cells, as compared to untreated cells. Microarray and quantitative real-time PCR (qRT-PCR) results showed that miR-103 decreased while the STAT1 expression increased substantially in RAW264.7 derived M1 macrophage, and there was a significant negative correlation between miR-103 and STAT1.Overexpression of miR-103 suppressed M1 polarization by inhibiting STAT1/IRF1 signaling pathway and vice versa in vitro. STAT1 is a direct target of of miR-103. RAW264.7 cells treated with LPS and IFNγ for 24h to generate M1-polarized macrophages. RAW264.7 (n= 3) and RAW264.7-derived M1 macrophages (n= 3) were used to evaluate miRNAs expression. RNA was isolated and miRNA array assays were performed by OE Biotech (mouse miRNA, release 21.0, 8*60K, Design ID: 070155))