Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Human Lung with Cell DIVE, v1.0

The OMAP Lung panel was designed for Cell DIVE imaging of Formalin-Fixed Paraffin-Embedded (FFPE) lung tissue. The<br>tissue slides were prepared with a two-step antigen retrieval process (pH 6 and pH 9, as described<br>[here](dx.doi.org/10.17504/protocols.io.bpwumpew). Antigens were detected using either primary antibodies with<br>fluorophore-labeled secondaries or fluorescent dye conjugated antibodies (protocol can be found<br>[here](dx.doi.org/10.17504/protocols.io.b4kgqutw). The panel contains 31 antibodies and the nuclear marker DAPI for<br>image alignment and nuclear segmentation. This OMAP provides a spatial context for key lung cell types in the [ASCT+B<br>lung table v1.1](https://doi.org/10.48539/HBM323.SGDF.945). <br>Additionally, the inclusion of protein biomarkers such as AGTR1, Cathepsin L, TMPRSS2, ICAM-1, and CD55 allow<br>characterization of proteins relevant to SARS-CoV-2 infection and profiling of the renin-angiotensin system (RAS) that<br>may play a role in COVID-19 disease. The core and essential protein biomarkers detailed here overlap (~25%) with the<br>panel developed for multiplexed imaging of lung samples established using conventional automated IHC staining for<br>FFPE-material (Dr. Christopher Werlein, an expert lung pathologist). This OMAP does not contain antibodies for<br>typing subsets of fibroblasts, macrophages, or endothelial cells.