RNAseq analysis of bone marrow plasma cells from fasted mice

Humoral immunological memory is essential for the protection against pathogens post primary infection or vaccination. This protection relies on the long-term survival of pathogen-specific memory B cells and lone-lived plasma cells. We found that fasting impairs the humoral immunological memory by depleting the plasma cells. We performed functional analysis of these bone marrow plasma cells from Ad Lib mice or fasted mice. Overall design: Mice were subjected to a prolonged fasting treatment (72 hours) or had free access to food, respectively. Then 10,000 plasma cells (CD138high CD267high) in bone marrow of these mice were sorted using BD FACSAria ?. mRNA profiles of sorted bone marrow plasma cells were generated in triplicates by Illumina Platform.