LncRNA/mRNA sequencing analysis on cerebral cortex of P301S mutant Tau transgenic mice (PS19).

Aggregation of the microtubule-associated protein, tau, can lead to neurofibrillary tangle formation in neurons and glia, the hallmark of tauopathy. The cellular damages induced by the tau overexpression and aggregation may lead to multiple pathologic features of tauopathy. However, the effect of aging on tauopathy has not been elucidated yet. Here, we conducted lncRNA/mRNA sequencing analysis on P301S mutant Tau transgenic mouse model (PS19) with different ages to track the genetic changes occurred by the aging and progression of tau overexpression. Overall design: P301S TG mice and their wild type mice were raised in animal facility at National University of Singapore. At 3 and 10 months-old age, both type of mice were sacrificed and cerebral cortex was dissected and kept frozen. Total RNA was extracted from the collected cerebral cortex tissues and lncRNA/mRNA sequencing was conducted using Illumina HiSeq 4000 system by Novogene.