Insulin fused to apolipoprotein A-I

In order to evaluate the in vivo properties of insulin fused to apolipoprotein A-I, we compared the pharmacodynamic properties in the liver to other insulin derivatives with long half-life in circulation: the fusion protein of albumin and insulin (Albulin) [30]. We administered subcutaneously saline, Albulin or Insulin-Apo to wild type mice, and 6 hours later, livers were extracted and homogenized. RNA was obtained and used to analyze the gene expression profile induced by the different insulin modifications. We administered subcutaneously saline, Albulin (55nmol/kg), Insulin-Apo (55nmol/kg) or insulin glargine (55nmol/kg) to wild type mice, and 6 hours later, livers were extracted and homogenized. RNA was obtained and used to analyze the gene expression profile induced by the different insulin modifications using Affymetrix Mouse Gene 1.0 ST array.