Data from: Effects of population structure and sex on association between serotonin receptors and Drosophila heart rate

As a first step toward population and quantitative genetic analysis of
neurotransmitter receptors in Drosophila melanogaster, we describe the
parameters of nucleotide variation in three serotonin receptors and their
association with pupal heart rate. Thirteen kilobases of DNA including the
complete coding regions of 5-HT1A, 5-HT1B, and 5-HT2 were sequenced in 216
highly inbred lines extracted from two North American populations in
California and North Carolina. Nucleotide and amino acid polymorphism is
in the normal range for Drosophila genes and proteins, and linkage
disequilibrium decays rapidly such that haplotype blocks are typically
only a few SNPs long. However, intron 1 of 5-HT1A consists of two
haplotypes that are at significantly different frequencies in the two
populations. Neither this region of the gene nor any of the common amino
acid polymorphisms in the three loci associate with either heart rate or
heart rate variability. A cluster of SNPs in intron 2 of 5-HT1A, including
a triallelic site, do show a highly significant interaction between
genotype, sex, and population. While it is likely that a combination of
weak, complex selection pressures and population structure has helped
shape variation in the serotonin receptors of Drosophila, much larger
sampling strategies than are currently adopted in evolutionary genetics
will be required to disentangle these effects.