Uptake of alpha-synuclein preformed fibrils is suppressed by inflammation and induces an aberrant phenotype in human microglia

To detect the microglial mRNA signature whilst phagocytosing alphasynuclein preformed fibrils (αSyn PFFs) with or without inflammatory stimulus, we treated the healthy control microglia with IFNγ (20ng/ml) 28h and αSyn PFFs (0.5µM) 4h Comparative gene expression analysis of RNA-seq data for human iPSC derived microglia treated with IFNγ 28h and αSyn PFFs 4h (n= 5-7 replicates/treatment, 1 male line, 1 female line)