Supplemental Material for Wagner et al., 2025

Laboratory cultures of cyanobacteria often have other bacteria growing with them that cannot be easily removed. When you isolate DNA from the cyanobacterium to sequence its genome, you also get DNA from the other bacteria too. As cyanobacteria are hard to extract DNA from, you sometimes get more DNA from the other bacteria than from the cyanobacterium. We realized that these problems might be able to solve each other, by repeating our DNA extraction on the same cells of cyanobacterium, we saw DNA from the other bacteria in the early fractions, with cyanobacterial DNA enriched in the later fraction.