Expression data from WT and AIMp1KO mouse bone marrow-derived dendritic cells. Mus musculus

Dendritic cells are critical regulators of adaptive immune responses which can process and present antigens to effector cells. As a structural component of multi-enzyme aminoacyl tRNA synthetase complex, AIMp1 (p43) is identified to promote type 1 T-cell responses by DCs and play important roles in both antitumor and antiviral immunity. We use high-throughput microarray analysis to compare the gene expression profiles of WT vs. AIMp1KO bone marrow-derived DCs (BMDC) to study the mechanism by which DC-expressed AIMp1 promote type 1 T-cell immune responses. Overall design: WT or AIMp1KO BMDCs were loaded with antigens and matured for RNA extraction and hybridization on Affymetrix microarrays. Cells were unloaded or loaded with OVA protein and SIINFEKL peptide (10ug/mL) for 3 hours in media containing 5% serum; then matured with cytokine cocktail (10ng/mL TNFa, 15ng/mL IL-6, 10ng/mL IL-1b, 1ug/mL PGE2) for 48hours prior to harvest.