CD4 T cells increase expression of several Th17-associated genes after three days of stimulation in a model of T cell-dependent B cell activationa.

aThe top ten induced genes as determined by quantitative RT-PCR in CD4 T and B cell populations purified by FACS from BT co-cultures stimulated for three days with α-IgM and SAg stimulation. Gene expression values (2−Ct) were normalized to b-actin gene expression and presented as the fold change average ± standard deviation of cells sorted from 3 independent donor pools of BT co-cultures compared to cells isolated from non-stimulated BT co-cultures.