Genome-wide analysis of brown adipose tissue gene expression of IL15-depleted mice under normal diet or diet enriched with the beta3-adrenergic agonist CL 316243

Chronic inflammation is one of the major players in the obesity related metabolic syndrome. However the various inflammatory mediators appear to promote insulin resistance directly or indirectly through their ability to induce the inflammatory cascade. Interleukin-15 (IL-15) is a pro-inflammatory cytokine that is involved in the pathogenesis of different autoimmune diseases such as rheumatoid arthritis, inflammatory bowel disease and type 1 diabetes. We postulated that as a pro-inflammatory cytokine, IL-15 promotes obesity during fat excess by promoting insulin resistance from tissues involved in energy metabolism. We used microarrays to characterize the gene expression profile of the brown adipose tissue of IL-15 mice under normal diet or diet enriched with the beta3-adrenergic agonist CL 316243 Total RNA obtained from adipose tissue of wt- or IL15-KO mice under normal diet or diet enriched with the beta3-adrenergic agonist CL 316243慢性炎症是肥胖相关代谢综合征的主要致病因素之一。各类炎症介质可通过诱导炎症级联反应，直接或间接促进胰岛素抵抗。白细胞介素-15（Interleukin-15，IL-15）作为一种促炎细胞因子，参与类风湿关节炎、炎症性肠病、1型糖尿病等多种自身免疫疾病的发病进程。我们提出如下假说：作为促炎细胞因子，IL-15可通过影响能量代谢相关组织的胰岛素抵抗，在脂肪过剩时促进肥胖的发生。本研究采用基因芯片（microarrays）技术，对正常饮食或添加β3-肾上腺素能激动剂CL 316243的饮食喂养的IL-15小鼠的棕色脂肪组织基因表达谱进行表征；本研究所用总RNA提取自正常饮食或添加β3-肾上腺素能激动剂CL 316243的饮食喂养的野生型（wt-）或IL-15敲除（IL15-KO）小鼠的脂肪组织。