Data from: A quantitative inventory of yeast P body proteins reveals principles of composition and specificity

P bodies are archetypal biomolecular condensates that concentrate proteins
and RNA without a surrounding membrane. While dozens of P body proteins
are known, the concentrations of components in the compartment have not
been measured. We used live cell imaging to generate a quantitative
inventory of the major proteins in yeast P bodies. Only 7 proteins are
highly concentrated in P bodies (5.1-15 uM); the 24 others examined are
appreciably lower (most ≤ 2.6 uM). P body concentration correlates
inversely with cytoplasmic exchange rate. Sequence elements driving Dcp2
concentration into P bodies are distributed across the protein and act
cooperatively. Our data indicate that P bodies, and probably other
condensates, are biochemically simpler than suggested by proteomic
analyses, with implications for specificity, reconstitution and evolution.