scRNA-seq data in ovarian cancer

Ovarian cancer (OC) is an aggressive gynecological tumour usually diagnosed at a late stage with widespread metastases and ascites. We depicted a single-cell landscape of OC ecosystem with five tumour-relevant sites: peripheral blood (PB), pelvic lymph node (PLN), primary tumour (Pri.OT), omentum metastasis (Met.Ome), and malignant ascites. Intercellular tissue dynamics of certain T cells, like GZMK+ effector memory T cell (Tem) and exhausted T cell (Tex), revealed potential roles of ascites as a pool for tumour-infiltrating T cells. Of the macrophages in tumours and ascites that exhibited distinct functional states, ascites-enriched macrophages were more of embryonic origin. For stromal cells in ascites, DES+ mesothelial cells account for the majority and help remodel its immune microenvironment. Further, we identified two endothelial subsets in primary tumours and one MAIT cluster in ascites predicting the responses to platinum-based chemotherapy of OC patients. Our study provides additional insights for ascites ecosystem and its connection with other tumour-relevant tissues, as well as potential markers for efficacy evaluation and therapies overcoming resistance in OC.

卵巢癌（Ovarian cancer, OC）是一类侵袭性妇科肿瘤，通常确诊时已处于晚期，伴随广泛转移与恶性腹水。本研究构建了卵巢癌生态系统的单细胞图谱，涵盖5个肿瘤相关样本位点：外周血（peripheral blood, PB）、盆腔淋巴结（pelvic lymph node, PLN）、原发肿瘤（primary tumour, Pri.OT）、网膜转移灶（omentum metastasis, Met.Ome）以及恶性腹水。针对特定T细胞（如GZMK+效应记忆T细胞（effector memory T cell, Tem）与耗竭性T细胞（exhausted T cell, Tex））的细胞间组织动态分析显示，腹水可作为肿瘤浸润T细胞的储备库，发挥潜在功能。肿瘤及腹水中的巨噬细胞呈现出迥异的功能状态，其中富集于腹水的巨噬细胞更多起源于胚胎谱系。针对腹水中的基质细胞，DES+间皮细胞占多数，并参与重塑腹水的免疫微环境。此外，本研究在原发肿瘤中鉴定出两种内皮细胞亚型，在腹水中鉴定出一个黏膜相关恒定T细胞（Mucosal-associated invariant T cell, MAIT）簇，该亚型可预测卵巢癌患者对铂类化疗的响应情况。本研究为腹水生态系统及其与其他肿瘤相关组织的关联提供了全新见解，同时也为卵巢癌的疗效评估及克服治疗耐药的治疗策略提供了潜在分子标志物。

搜集汇总

背景与挑战

背景概述

该数据集提供了卵巢癌患者五个肿瘤相关部位的单细胞RNA测序数据，揭示了腹水生态系统的特殊作用和潜在化疗反应标志物，数据来自14名患者并使用标准化流程处理。