Transcriptome analysis of different tissues in different materials

We used two hybrid combinations and their 4 parents as materials. One week after topping the tobacco plant, samples were taken, including three tissues: root, stalk, and leaf. The RNA of these samples was extracted, and transcriptome sequencing analysis was carried out to explore the transcriptional profile differences between hybrids and their parents, and to analyze the gene differential expression between different tissues. Fifty-four samples were analyzed, including six plant materials,three plant tissues, each with three replicates.