Next generation sequencing analysis of mouse adrenal glands after a one-hour dexamethasone treatment

RNA sequencing (RNA-seq) was used to identify early transcriptomic responses of the adrenal gland to the 1-hour dexamethasone treatment in mice. Overall design: Five male mice were IP injected with dexamethason (10 micro gram) and another five mice were IP injected with phosphate-buffered saline. Adrenal glands were collected one hour after the treatment. Adrenal glands were pooled (2 mice and 3 mice) and total RNAs were extracted to make two replicates for each treatment group. Paired-end RNA-seq (PE150) was performed using the Illumina platform.