Rigidity of cell fate and function among monocytes (ATAC-Seq). Rigidity of cell fate and function among monocytes (ATAC-Seq)

Here, we used an inducible and reversible system to pause myeloid differentiation at a self-renewing state. These cells were clonally isolated and allowed to differentiate to test whether function was clone-specific and clonally restricted. We found that monocytic subsets exist with inherent, restricted capabilities which differ in their capacity to perform traditional monocytic functions. Overall design: We performed high-throughput ATAC-sequencing on both progenitors and monocyte ER-Hoxb8 clones, clonally paired as progenitors and mature cells.