Aiming for ultra-long read lengths in Drosophila melanogaster to facilitate genome assembly and structural variation studies and tool development. If you use this data, please cite: Sequencing ultra-long DNA molecules with the Oxford Nanopore MinION John M Urban, Jacob Bliss, Charles E Lawrence, Susan A Gerbi doi: https://doi.org/10.1101/019281 https://www.biorxiv.org/content/early/2015/05/13/019281

A protocol similar to protocols presented in the BioRxiv preprint by John Urban et al entitled, Sequencing Ultra-Long DNA Molecules with the Oxford Nanopore MinION was used (circa May 2015) with Oregon-R Drosophila melanogaster embryos for a single flow cell of MinION sequencing. This dataset includes some very long 2D reads (up to 97 kb) that align to the reference with similar error-rates as reported for MinION sequencing in general. The flow cell output included (assuming 150 Mb genome): 27.8 Mb of 2D reads (~0.185x), 82 Mb of template (~0.55x), and 38.5 Mb complement (~0.257x). BLASR alignment to dm6 (including all the unplaced contigs): 2D mean coverage = ~0.14x, Template mean coverage = ~0.17x, and Complement mean cov = ~0.10x. LAST alignment: 2D mean coverage = ~0.10x, Template mean cov = ~0.12x, Complement mean cov = ~0.08x. This data was generated by John Urban for the MARC consortium, and was of particular interest to those interested in genome assembly of larger more complex genomes (than E. coli) and those interested in structural variation studies. John can generate more Drosophila data if it is chosen for MARC Phase 3.