MouseLight Neuron AA1604

A vast neural tracing effort by a team of Janelia scientists has upped the number of fully-traced neurons in the mouse brain by a factor of 10. Researchers can now download and browse the data in three dimensions. Inside the mouse brain, individual neurons zigzag across hemispheres, embroider branching patterns and, researchers have now discovered, can even spool out spindly fibers up to 45 centimeters long. Scientists can see and explore these wandering neural traces in 3-D in the most detailed map of mouse brain wiring yet created. The map reconstructs the shape and position of more than 300 of the 70 million neurons in the mouse brain. Previous efforts to trace the path of individual neurons had topped out in the dozens. The selectively-labeled neurons were mapped in an iterative process with two-photon microscopy. The brain is sliced in 200-micron sections and a few dozen neurons are labeled at a time and imaged. Each brain imaged yields about 50 terabytes of data, each containing mapped neurons which can be browsed via the NeuronBrowser application at https://ml-neuronbrowser.janelia.org/. Researchers interested in the complete raw data set should contact Janelia to discuss obtaining it via hardware transfer.

由 Janelia 科学家团队发起的一项庞大的神经追踪工作，将小鼠大脑中完全追踪到的神经元数量提高了十倍。研究人员现在可以下载并三维浏览这些数据。在小鼠大脑内部，单个神经元在半球之间蜿蜒曲折，编织出分支图案，而研究人员最近发现，这些神经元甚至可以延伸出长达 45 厘米的细长纤维。科学家们可以在迄今为止创建的小鼠大脑连接最详细的图谱中，观察并探索这些游走的神经轨迹。该图谱重建了小鼠大脑中 7000 万个神经元中超过 300 个的形状和位置。先前追踪单个神经元路径的努力已达到数十个神经元的极限。通过迭代过程和双光子显微镜，对这些选择性标记的神经元进行了映射。大脑被切成 200 微米的薄片，每次标记并成像的神经元数量仅有数十个。每张成像的大脑产生约 50 太字节的数据，每份数据中都包含可以浏览的映射神经元，通过 NeuronBrowser 应用程序（https://ml-neuronbrowser.janelia.org/）进行浏览。对完整原始数据集感兴趣的研究人员应与 Janelia 联系，讨论通过硬件传输获取数据的方式。