Expression data from Balb/c mice kidney samples

Males are 50% more likely to develop end stage kidney failure compared to women. As a model of the human condition we analyzed gene expression changes in healthy and diseased mouse kidneys. Interestingly, the set of gender biased genes in healthy kidneys were different from those in diseased kidneys indicating not only baseline gene expression differences but also that the male and female kidney respond differently to disease condition. Keywords: Gender difference We used AffymetrixM430A2.0 microarrays to determine global gene expression differences in kidneys of 10-week old healthy Balb/c male and female mice. In order to identify gene expression differences under disease conditions, animals were injected with doxorubicin, a substance that is known to induce albuminuria and glomerulosclerosis in Balb/c mice as it is directly toxic to podocytes.与女性相比，男性罹患终末期肾衰竭的风险高出50%。作为模拟人类疾病状态的动物模型，我们分析了健康与患病小鼠肾脏中的基因表达变化。值得注意的是，健康肾脏中的性别偏向基因集与患病肾脏中的存在显著差异，这不仅表明基线基因表达存在性别差异，同时也说明雌雄肾脏对疾病状态的应答模式存在不同。 关键词：性别差异 我们使用Affymetrix M430A2.0基因芯片，对10周龄健康Balb/c雌雄小鼠的肾脏开展全基因组基因表达差异分析。为鉴定疾病状态下的基因表达差异，我们向小鼠注射了阿霉素（doxorubicin）——该物质可对足细胞产生直接毒性，已知会在Balb/c小鼠中诱导蛋白尿与肾小球硬化症。