Localization Precision Analysis Data for Interferometric Single-Molecule Localization Microscopy

This dataset contains measurement data of localization precision using single-molecule interferometric localization microscopy. The localization precision of the single-molecule interferometric localization microscope was measured using 40 nm fluorescent microspheres. This dataset includes raw image files, data result files processed with MATLAB, and result images. The results show localization precisions of 6.6 nm, 6.4 nm, and 4.7 nm for the X, Y, and Z directions respectively, achieving isotropic single-molecule localization precision and uniform resolution in all directions.<b>File description:</b><b>IntComp.mat:</b> Brightness calibration data<b>pixComp.mat:</b> Pixel calibration data<b>RegInfo.mat:</b> Raw image cropping and transformation data<b>spool_A_1_MMStack_Default.ome.tif:</b> Camera A data<b>spool_A_2_MMStack_Default.ome.tif:</b> Camera B data<b>spool_A_1_MMStack_Default.ome_result.mat:</b> Processed result data file<b>spool_A_1_MMStack_Default.ome_result_sel.png:</b> Illustration of selected fluorescent microspheres<b>spool_A_1_MMStack_Default.ome_result1.png:</b> Summary of processing results including XY localization error, brightness, phase error, modulation depth, etc.<b>spool_A_1_MMStack_Default.ome_result2.png:</b> XYZ localization precision analysis result image