Human Microbiome Project (HMP) 16S rRNA Gene Diversity, the diversity of 16S ribosomal RNA genes in the human microbiome: 454 Protocol Development - Mock

The HMP early mock pilot represents the pyrosequencing of 16S rRNA genes amplified from HMP even and staggered Mock communities, distributed to each of the four HMP sequencing centers. This pilot was conducted using a pre-production version of the 454 sequencing protocol which has since been deprecated. The main differences with the production protocol are: no use of barcodes, bi-directional sequencing, pools containing 3 different 16S windows (V1-V3, V3-V5, V6-V9). (Previously referred to as Pre-CEFoS Mock Pilot)