Conventional DC1 (cDC1) are Strongly Enriched, Quiescent and Relatively Tolerogenic in Local Inflammatory Arthritis

We sequenced (bulk RNA-seq) conventional (c)DC1, cDC2 and monocytes from a local inflammatory site, namely synovial fluid (SF) from patients suffering from a chronic inflammatory condition, Juvenile Idiopathic Arthritis (JIA). To be able to compare to steady state, we also sequenced cDC2 and monocyte subsets from peripheral blood of healthy controls. We isolated these antigen-presenting cells from synovial fluid by FASCsort, and then analysed gene expression using data from RNA-seq of n=4 patients, 3 cell types each, and n=4 healthy controls, 3-4 cell types each. ------------------------------------- Authors state "The raw RNA-seq data from the cohort are not publicly available due to research participant privacy/consent."